Early postoperative changes in serum Isthmin levels after laparoscopic sleeve gastrectomy: A prospective cohort study
Onur Ağ1, Nizamettin Kutluer1, Mehmet Zeki Öğüt1, Ayşe Azak Bozan2, Akan Yeniçerioğlu1, Hakan Ayyıldız3, Mehmet Buğra Bozan11Turkish Ministry of Health, Medical Sciences University, Fethi Sekin City Hospital, Elazig, Türkiye2Turkish Ministry of Health, Department of Anesthesiology and Reanimation, Medical Sciences University, Fethi Sekin City Hospital, Elazig, Türkiye
3Turkish Ministry of Health, Department of Biochemistry, Medical Sciences University, Fethi Sekin City Hospital, Elazig, Türkiye
INTRODUCTION: The primary objective of this study was to examine changes in serum adipokine Isthmin levels before and after surgery following weight loss, to determine its potential as a biomarker in this patient population, and to evaluate the relationship between reduced adipose tissue and adipokines after obesity surgery.
METHODS: Preoperative complete blood count (CBC) and biochemical values, as well as post-operative third-month CBC and biochemical values, were recorded through a prospective chart review. Pre-operative and postoperative height and weight measurements were obtained, and body mass index (BMI), excess weight loss, and total weight loss were calculated. Subsequently, a 5 mL blood sample was collected at presentation for measurement of serum Isthmin levels (BD Vacutainer, Plymouth, UK). The samples were centrifuged at 3500–4000 rpm for 10 min, transferred into Eppendorf tubes, and stored at −20°C until analysis. Preoperative and third-month postoperative serum Isthmin levels were then measured and recorded.
RESULTS: No statistically significant difference was observed between preoperative and postoperative serum Isthmin levels. However, statistically significant differences were observed in body weight and BMI values (p=0.138, p<0.001, and p<0.001, respectively). When stratified by BMI groups, no statistically significant difference was observed in the change in Isthmin levels between the preoperative and postoperative periods.
DISCUSSION AND CONCLUSION: Although a correlation between Isthmin, an adipokine, and weight loss was hypothesized, no statistically significant difference was found. Longer-term studies are required before adipokines can be used as reliable biomarkers. Isthmin appears to have a weaker relationship with adipose tissue and weight loss and may not be directly associated with obesity; however, these novel molecules are still considered to hold potential for future biomarker development.
Keywords: Adipokine, isthmin, obesity
Onur Ağ1, Nizamettin Kutluer1, Mehmet Zeki Öğüt1, Ayşe Azak Bozan2, Akan Yeniçerioğlu1, Hakan Ayyıldız3, Mehmet Buğra Bozan11
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Manuscript Language: English